Blog Post Week 2

This week for the pseudomonas project we will be doing gel electrophoresis on our samples. We have done water and biofilm collection. We moved on to do filtration, using a filtration apparatus we trapped any bacteria onto a filter. That filter was then transferred onto a plate to allow for us to culture the bacteria. The growth was then transferred into broth to allow to proceed into DNA extraction. For the DNA extraction we follow a protocol. I found this protocol interesting because we can see the chromosomal DNA threads being formed. We moved on to Nano drop to see the DNA concentration. Once we finished Nano dropping we moved on to PCR. What PCR does is amplifies our DNA using primers that are specific to the pseudomonas we are looking for. Next week we hope to have our gel electrophoresis results and maybe move on to DNA sequencing.